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KMID : 1094720150200061123
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Biotechnology and Bioprocess Engineering
2015 Volume.20 No. 6 p.1123 ~ p.1132
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Semi-solid state fermentation of food waste for production of Bacillus thuringiensis biopesticide
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Zhang Wenyu
Zou Hui
Jiang Lin
Yao Juejun
Liang Jing
Wang Qunhui
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Abstract
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In this study, Bacillus thuringiensis (Bt) biopesticide was produced through different fermentation processes using food waste with different water contents. The semi-solid state sample with 75% water content presented the highest δ-endotoxin efficiency of 862 μg/mL. δ-endotoxin efficiency increased by 30.2% from that of the solid-state sample (50% water content) and 73.8% from that of the submerged sample (99% water content). Results confirmed that semi-solid state fermentation presents considerable advantages compared with other fermentation types (solid-state and submerged). The timing adjustment of pH and recycling fermentation effectively counteracted the inhibition of pH and product, thereby increasing δ- endotoxin efficiency to 1,648 and 2,478 μg/mL (accumulated value of all four fermentation loops), respectively. A δ- endotoxin slow-release formulation using polylactic acid as the carrier was also developed to effectively promote the stability of Bt biopesticide.
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KEYWORD
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Bacillus thuringiensis, food waste, semi-solid state fermentation, biopesticide, slow-release formulation, δ-endotoxin
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